• Anti-YFP antibody at 1:2000 dilution; 293HEK cells transduced with YFP Ad; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution
  • Immunofluorescence – anti-YFP antibody in 293HEK cells transfected with YFP at 1:50 dilution; cells were fixed with 4% of PFA
  • Immunogold labeling of epithelium cells, in vivo injected with YFP expressing vector

Anti-YFP antibody (STJ140118)

SKU:
STJ140118

Current Stock:
Host: Goat
Applications: WB/IHC-F/IHC-P/IF/IEM
Reactivity: Transfected cells
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Goat polyclonal antibody anti-Yellow Fluorescent Protein is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immune Electron Microscopy research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS, 20% Glycerol and 0.05% Sodium Azide.
Purification: This antibody is epitope-affinity purified from goat antiserum.
Concentration: 3 mg/ml
Dilution Range: WB 1:500-1:2000
IHC-F 1:50-1:1000
IHC-P 1:50-1:1000
IF 1:50-1:1000
IEM 1:50-1:1000
Storage Instruction: For continuous use, store at 2-8 C for one-two days. For extended storage, store in-20 C freezer. Working dilution samples should be discarded if not used within 12 hours.
Specificity: In 293HEK cells transfected with cds plasmid detects a band of 27 kDa by Western blot. This antibody also recognizes GFP and does not recognize mCherry/red fluorescent protein.
Immunogen: Purified recombinant peptide produced in E. coli.
Background Goat polyclonal antibody to YFP (yellow fluorescent protein). YFP is a genetic mutant of green fluorescent protein (GFP) , derived from Aequorea victoria. Its excitation/emission peaks are 514/527 nm respectively.

Information sourced from Uniprot.org

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